饲粮蛋氨酸对鹅再生羽绒品质、血清指标及基因mRNA表达量的影响

doi:10.11843/j.issn.0366-6964.2019.02.010

摘要/Abstract

摘要：

旨在探讨饲粮蛋氨酸对鹅羽绒再生性能、血清指标及半胱氨酸和蛋氨酸代谢通路相关基因表达量的影响。本试验以128只健康霍尔多巴吉鹅为研究对象，于150日龄采绒后随机分成4组，每组4个重复，每个重复8只鹅。各组试验鹅分别饲喂基础饲粮中蛋氨酸添加量为0、0.28%、0.56%和0.84%的饲料，正试期42 d。测定比较试验鹅的羽绒千朵绒重、羽枝长度以及血清生化、抗氧化和促生长因子等指标，并通过RT-qPCR检测半胱氨酸和蛋氨酸代谢通路相关基因mRNA的相对表达量。结果发现：1）随着饲粮中蛋氨酸添加比例增加，鹅血清中总蛋白（TP）、白蛋白（ALB）、尿素氮（BUN）、三酰甘油（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）含量等均未发生显著变化（P>0.05）；血清中促生长因子（IGF-1）、催乳素（PRL）和谷胱甘肽过氧化物酶（GSH-Px）浓度随之显著升高（P<0.05），但添加量达到0.84%时反而又降低。2）饲粮中添加蛋氨酸可显著提高羽绒的千朵绒重和背部羽枝长度（P<0.05），促进羽绒再生，但不同蛋氨酸添加浓度对羽绒产量影响的差异不显著（P>0.05）。3）RT-qPCR检测结果显示，饲粮中添加蛋氨酸，半胱氨酸和蛋氨酸代谢通路中MAT1A mRNA相对表达量显著下调（P<0.05），基因AHCY在蛋氨酸添加比例为0.28%时mRNA相对表达量极显著下调（P<0.01）。结果表明，饲粮中添加适当浓度的蛋氨酸，可促进鹅体内IGF-1和PRL等促生长因子和抗氧化因子分泌，还可显著提高千朵绒重、背部羽枝长度，降低半胱氨酸和蛋氨酸代谢通路中MAT1A和ACHY基因mRNA的相对表达量，促进羽绒再生，其适宜的总添加量为0.55%~0.83%。

Abstract:

The aim of this study was to investigate the effects of dietary methionine on the regeneration performance of goose down, serum indexes and the expression of genes related to cysteine and methionine metabolic pathways in Hortobagy goose (Goosebag goose). One hundred and twenty eight 150-day-old healthy Hortobagy geese were selected and randomly divided into 4 groups after epilation. Four replicates for each group, and 8 geese for each replicate. Geese in the 4 groups were fed diets with additional methionine levels of 0, 0.28%, 0.56% and 0.84%, respectively. The formal experimental period was 42 days. The data of goose down such as the thousand plush weight,velvet length, serum biochemical indicators, growth factors and antioxidant factors levels were measured, and the expression levels of genes related to cysteine and methionine metabolism pathways were determined by RT-qPCR. The results showed as the following:1) There was no significant difference in the TP,ALB,BUN,TG,HDL and LDL levels in serum among different groups (P>0.05). At the same time, with the increase of dietary methionine concentration, the growth factor (IGF-1), prolactin(PRL) and the glutathione (GSH-Px) levels in serum were also significantly increased(P<0.05), but when the methionine level added to 0.84%, the concentration of the above factors reduced. 2) Supplementation of methionine in diet could significantly increase the thousand down weight and velvet length of back(P<0.05), and promote the regeneration of down, but there was no significant difference in the yield of down among the different methionine supplementation groups(P>0.05). 3) The results of RT-qPCR showed that, with the methionine addition, the mRNA expression of MAT1A gene in cysteine and methionine metabolism pathway decreased significantly (P<0.05), and the expression of ACHY mRNA extremely significantly decreased in the 0.28% methionine supplementation group(P<0.01). The results indicate that suitable concentration of methionine in diet can promote the secretion of IGF-1 and PRL growth factors and antioxidant factors in goose. It can also significantly increase the thousand plush weight and velvet length of back, reduce the mRNA expression of the MAT1A and ACHY genes in cysteine and methionine metabolism pathways and promote the regeneration of the down feather, and the suitable total addition level is 0.55%-0.83%.

中图分类号:

S835.5

王惠影, 郭保地, 何大乾, 龚绍明, 刘毅, 李光全, 徐琪, 陈国宏. 饲粮蛋氨酸对鹅再生羽绒品质、血清指标及基因mRNA表达量的影响[J]. 畜牧兽医学报, 2019, 50(2): 323-331.

WANG Huiying, GUO Baodi, HE Daqian, GONG Shaoming, LIU Yi, LI Guangquan, XU Qi, CHEN Guohong. Effect of Dietary Methionine on Down Quality of Regeneration, Serum Indexes and Gene Expressions in Goose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 323-331.

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